Contact an Account ManagerCombined HPLC and enzyme immunoassay for active metabolites of a sleep inducer (rilmazafone) a ring-opened derivative of benzodiazepines in human plasma
Summary
Type: HPLC
Application #: 105220
Catalog#: 720014.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x250mm
Detection: enzyme immunoassay and UV, 254nm
Conditions: eluent: acetonitrile - water, 13min isocratic 35:65 (v/v), then exponential gradient to 100% acetonitrile in 12min
flow rate 1ml/min
Authors: Kominami G
Source: J Chromatogr 417 (1987) 216 - 222
Details
Legend: Fig. 3. Combined HPLC-EIA of a human plasma sample 4h after oral administration
of 2mg of the parent drug. UV traces (top right) were obtained from this sample
(dashed line) and from authentic sampl